Sedimentation Velocity Studies of Human Methaemoglobin Modified by Gamma Radiation
نویسندگان
چکیده
منابع مشابه
Osmotic pressures and sedimentation velocity of Gastrophilus methaemoglobin.
In the preceding paper, Keilin & Wang (1946) have described the preparation and properties of the haemoglobin obtained from the tracheal cells of the larva of Gastrophilus (the bot fly). Samples from three of their preparations in the form ofmethaemoglobin were used for measurements of osmotic pressure. The amount of material available was small and the results recorded must be regarded as prov...
متن کاملMechanism of methaemoglobin reduction by human erythrocytes.
The time course of methaemoglobin reduction in human erythrocytes treated with nitrite was studied at pH 7.4, 37 degrees C, in the presence or absence of Methylene Blue, and the changes in methaemoglobin, intermediate haemoglobins and oxyhaemoglobin during the reaction were analysed by isoelectric-focusing on Ampholine/polyacrylamide-gel plates. In both cases, with or without the dye, the inter...
متن کاملAnalytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium.
Analytical ultracentrifugation (AUC) is a versatile and powerful method for the quantitative analysis of macromolecules in solution. AUC has broad applications for the study of biomacromolecules in a wide range of solvents and over a wide range of solute concentrations. Three optical systems are available for the analytical ultracentrifuge (absorbance, interference, and fluorescence) that permi...
متن کاملSedimentation Velocity Analytical Ultracentrifugation
(b) Sedimentation equilibrium: an experiment performed at a lower speed so that the sedimentation and backdiffirsion forces are of comparable magnitudes and therefore give rise to an equilibrium distribution of solute concentration. Because there is no net transport at equilibrium, shape effects do not come into play and the distribution becomes an absolute function of molecular mass for a sing...
متن کاملMethaemoglobin Content and NADH-Methaemoglobin Reductase Activity of Three Human Erythrocyte Genotypes
Background: To study methaemoglobin content and NADH-methaemoglobin reductase activity of three human erythrocyte genotypes (HbAA, HbAS and HbSS).Materials and Methods: Studies to ascertain methaemoglobin concentration and level of NADH-methaemoglobin reductase activity of three human erythrocyte genotypes (HbAA, HbAS and HbSS) were carried out in forty-three (43) healthy male participants of c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine
سال: 1981
ISSN: 0020-7616
DOI: 10.1080/09553008114550821